ENTERPRISE AI ANALYSIS
Behavioral dataset for Long-Evans and its schizophrenia-like substrain through several generations
We present a high-throughput behavioral dataset acquired with Ambitus, an automated reward-based corridor system that records locomotor and exploratory activities and cognitive functions after minimal handling. The collection contains 91 raw and derived variables, each measured across four consecutive trials, for 1,342 Long-Evans rats, including a triple-hit schizophrenia-like substrain (Lisket) bred through 16 generations. All data files, detailed metadata and analysis scripts are openly available on Zenodo. This resource enables longitudinal and multivariate studies of behavioral phenotypes, trans-generational effects, and strain differences, and it provides a benchmark for machine-learning-based marker discovery in rodent models.
Key Metrics & Impact
Leveraging automated behavioral tracking and multi-generational breeding, this dataset offers unparalleled depth for AI-driven psychiatric research.
1,342
Total Rats Studied
16
Generations Bred
91
Variables Recorded
>360,000
Individual Data Points
Deep Analysis & Enterprise Applications
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Overview & Significance
We present an openly available, comprehensive, multi-generation behavioral dataset for a schizophrenia-like rat model. The dataset comprises 1,342 Long-Evans and Lisket rats tested across 16 generations (2019-2025). Ninety-one parameters describing locomotion, exploration, reward collection and learning were recorded in four sequential trials per animal with the Ambitus corridor system, yielding > 360,000 individual data points.
This resource enables longitudinal and multivariate studies of behavioral phenotypes, trans-generational effects, and strain differences, and it provides a benchmark for machine-learning-based marker discovery in rodent models.
Schizophrenia is a complex mental disorder... A "triple-hit" rat substrain, termed Lisket has been developed from the Long-Evans (LE) rats... through 16 generations (G0-G15).
Methodology
Animal Care & Ethics: Long-Evans, and Lisket rats in both sexes during a 7-year period were involved in the study. Animals were treated in accordance with the guidelines set by the Government of Hungary for the humane treatment of animals. All experiments were approved by the Hungarian Ethical Committee for Animal Research (RN: XIV/1248/2018 and XIV/1421/2023) and conducted in compliance with the EU Directive 2010/63/EU and the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines 2.0.
Ambitus Apparatus: The Ambitus apparatus, designed as a reward-based cognitive test, consists of a rectangular corridor constructed of clear Plexiglas on a black floor. It is fitted with infrared LED (light emitting diodes) and photocells to measure nose-poking activity (as exploration) in the boxes, the collection of rewards, and locomotor activity, respectively, with 1 ms time resolution.
Behavioral Test Protocol: At 10 weeks of age, animals underwent the Ambitus test with food restricted 48h prior. Two tasks were employed: Task 1 (Trials 1-2, all boxes filled with reward) in the morning, and Task 2 (Trials 3-4, only inside boxes baited) performed 3 hours later. This allowed determination of trial-dependent changes for 91 parameters.
Data Characteristics
Data Records: The dataset is provided in two files: a raw trial-level Parquet file containing detailed within-subject behavior, and a processed ML-ready CSV file with one row per animal summarizing behavioral performance across four Ambitus trials. Detailed metadata and analysis scripts are included.
Availability & Licensing: All data files, detailed metadata and analysis scripts are openly available on Zenodo (DOI: 10.5281/zenodo.16414790) under a CC-BY 4.0 licence, ensuring open access and reproducibility.
Data Completeness: Eighty-three of the 91 variables show <1% missing values, with 55 being fully complete. The limited missingness is protocol-driven (e.g., related to an animal not visiting a box), well documented, and evenly distributed across the 16 generations, satisfying FAIR "quality and reusability" criteria.
1,342
Total Long-Evans and Lisket Rats Studied
Enterprise Process Flow
Post-weaning Social Isolation
→
Subchronic Ketamine Treatment
→
Selective Breeding (16 Generations)
| Feature | Ambitus Apparatus (This Study) | Traditional Automated (e.g., Touch screen) | Other Mazes (e.g., Morris Water-maze) |
|---|---|---|---|
| Throughput | High | Low | Medium |
| Behavioral Detail | Rich (locomotor, exploratory, cognitive functions) | Rich (specific cognitive tasks) | Moderate (spatial memory, general activity) |
| Learning Sessions Required | Minimal handling, rapid acquisition | Intensive, long-lasting | Simple to moderate |
| Automation Level | Automated recording & analysis | Automated task delivery, some manual recording | Semi-automated (requires experimenter intervention) |
| Data Output | Rapid, quantitative behavioral readouts within minutes | Quantitative, detailed task performance | Mixed (qualitative observations, quantitative metrics) |
FAIR Data Practices for Enhanced Reproducibility
The full seven-year dataset is released in a FAIR format, providing comprehensive raw and processed files plus detailed metadata. This enables researchers to estimate heritability across generations, identify behavioral markers, validate translational models of schizophrenia, and benchmark machine-learning pipelines.
The scale of the dataset further supports pharmacological or toxicological screening, as well as studies of age-related cognitive decline and neural plasticity. Usage examples, step-by-step loading instructions and baseline analysis notebooks accompany the archive to facilitate immediate reuse. These materials furnish an open, extensible platform for behavioral neuroscience and data-driven discovery.
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